Vladimir Brukhin


Leading Researcher

In February 2013 Vladimir Brukhin Joined the Theodosius Dobzhansky Center for Genome Bioinformatics after returning from the UK, where he worked as a Senior Research fellow at the School of Biology, University of Edinburgh. His main research interests are in the field of functional genetics, comparative genomics, epigenetics, sexual and asexual plant reproduction, developmental biology and evolution. Currently Dr. Brukhin coordinates the project Genome Russia (http://genomerussia.bio.spbu.ru/?lang=en) main goal of which is creation of the web-based database containing depersonalised information on the whole-genome sequences of at least 2,500 men and women originating from the different regions of Russia, whose ancestors were indigenous to the region for several generations, as well as description of the genome variations in these groups, detection of the features that affect the spread of diseases and creation of a database of medically-relevant genomic variants characteristic to the Russian population. Other projects supervised by Dr. Brukhin are carried out in the frames of 3 year Swiss-Russian collaboration program “Sequencing, Assembly, and Annotation of the Highly Heterozygous Genome of the Apomictic Plant Boechera divaricarpa” and 2-year Russian-Indian program “Fixing hybrid vigor by asexual reproduction-A chemical genetics screen towards harnessing apomixis in flowering plants”. Main goal of the both projects is a better understanding of the molecular basis of apomixis the asexual reproduction of plants through seed, that could become a key ‘enabling technology’ of immense benefit to agriculture, because it would enable the permanent fixation of heterosis in crop plants. Also Dr. Brukhin develops the project “Whole genome methylation analysis in species-specific phenotypes and at the various developmental stages”.

Dr. Brukhin received his Master in Agricultural Chemistry in 1988 from the faculty of Biology and Soil Science, St. Petersburg State University. In 1993 he earned a Ph.D. in Developmental Biology from Komarov Botanical Institute, Russian Academy of Sciences. In 1994 – Dr. Brukhin worked as a trainee at the University of Lublin in Poland where he studied plant developmental biology and microscopy. In 1997-1999 He was a postdoctoral researcher in France and Sweden studying somatic embryogenesis, apoptosis and genes specifically expressed during and after fertilization. In 2000-2006 he worked as a Senior Research Associate at the Department of Plant Developmental Genetics, University of Zurich, Switzerland, where he was responsible for co-ordinating the lab contributions to the multinational EU project EXOTIC (Exon Trapping Insert Consortium) and also he developed an independent research project ‘The Ubiquitin 26S Proteasome Proteolytic Pathway and Plant Reproduction’. At the University of Zurich Dr. Brukhin was a practical instructor for Mittlebaukurs, Plant Developmental Biology. In 2006-2007 he was invited to the University of Illinois at Urbana-Champaign to participate in the project on developmental aspects of posttranslational protein modifications. In 2008-2013 Dr. Brukhin worked in the UK (University of Aberystwyth and University of Edinburgh) where he studied epigenetics, problems of plant cell identity and specialization. In the UK he was also involved in tuition and supervision of undergraduate and PhD students.

List of publications:

  1. Oleksyk TK, Brukhin V, O’Brien SJ. (2015) Putting Russia on the genome map. Science. 13; 350 (6262): 747.

  2. Oleksyk TK, Brukhin V, O’Brien SJ. (2015) The Genome Russia project: closing the largest remaining omission on the world Genome map. Gigascience; 4:53.

  3. Dobrynin P, Liu S, Tamazian G, Xiong Z, Yurchenko AA, Krasheninnikova K, Kliver S, Schmidt-Küntzel A, Koepfli KP, Johnson W, Kuderna LF, García-Pérez R, Manuel Md, Godinez R, Komissarov A, Makunin A, Brukhin V, Qiu W, Zhou L, Li F, Yi J, Driscoll C, Antunes A, Oleksyk TK, Eizirik E, Perelman P, Roelke M, Wildt D, Diekhans M, Marques-Bonet T, Marker L, Bhak J, Wang J, Zhang G, O’Brien SJ. (2015) Genomic legacy of the African cheetah, Acinonyx jubatus. Genome Biol.; 16:277.

  4. Tamazian G, Simonov S, Dobrynin P, Makunin A, Logachev A, Komissarov A, Shevchenko A, Brukhin V, Cherkasov N, Svitin A, Koepfli KP, Pontius J, Driscoll CA, Blackistone K, Barr C, Goldman D, Antunes A, Quilez J, Lorente-Galdos B, Alkan C, Marques-Bonet T, Menotti-Raymond M, David VA, Narfström K, O’Brien SJ.
    (2014) Annotated Features of Domestic Cat-Felis Catus. Gigascience. 3:13

  5. Brukhin V. and Morozova N. (2011) Plant growth and development – basic knowledge and current views. Mathematical Modelling Natural Phenomena, 6 (2): 1- 53.

  6. Brukhin V., Jaciubek, M., Bolaños Carpio A., Kuzmina V., Grossniklaus U. (2011) Female Gametophytic mutants of Arabidopsis thaliana identified in a gene Trap Insertional Mutagenesis Screen, Int. J. Dev. Biol. 55: 73-84.

  7. Dumbliauskas E., Lechner E., Alioua M., Cognat V., Brukhin V., Berger F., Koncz C., Grossniklaus U., Molinier J., Genschik P. (2011) The Arabidopsis CUL4-DDB1 complex interacts with MSI1 and is required to maintain MEA parental imprinting. EMBO J. 30 (4):731-43.

  8. Chekanova J.A., Gregory B.D., Reverdatto S.V., Chen H., Kumar R., Hooker T., Junshi Yazaki J., Li P., Skiba N., Peng Q., Alonso J., Brukhin V., Grossniklaus U., Ecker J.R.; Belostotsky D.A. (2007) Genome-Wide High-Resolution Mapping of Exosome Substrates Reveals Hidden Features in the Arabidopsis Transcriptome. Cell, 131 (7):1340-1353.

  9. Brukhin V., Curtis M.D., Grossniklaus U. (2005) The angiosperm female gametophyte: no longer forgotten generation. Review Current Science, 89 (11).

  10. Brukhin V., Gheyeselinck J., Gagliardini V., Genschik P., Grossniklaus U. (2005) The RPN1 subunit of the 26S proteasome in Arabidopsis is essential for embryogenesis. The Plant Cell, 17 (10): 2723-37.

  11. Thomann A. *, Brukhin V., Dieterle M., Gheyeselinck J., Grossniklaus U. and Genschik P. (2005) Arabidopsis CUL3A and CUL3B genes are essential for normal embryogenesis. The Plant Journal, 43 (3): 437-448.

  12. Brukhin V., Hernould M., Gonzalez N., Chevalier C., Mouras A. (2003) Flower development schedule in tomato Lycopersicon esculentum cv. sweet cherry. Sex. Plant Reproduction. 15 (6): 311-320.

  13. Brukhin V., Clapham D., Elfstrand M., von Arnold S. (2000) Basta tolerance as a selectable and screening marker for transgenic plants of Norway spruce.- Plant Cell Reports, 19: 899-903.

  14. Filonova L.H., Bozhkov P.V., Brukhin V.B., Daniel G., Zhivotovsky B. and von Arnold S. (2000) Two waves of programmed cell death occur during formation and development of somatic embryos in the gymnosperm Norway spruce. J Cell Sci, 113 (24): 4399-4411.

  15. Brukhin V.B., Moleva I.R., Filonova L.H., Grakhov V.P., Blume Ya.B, Bozhkov P.V. (1996) Proliferative activity of callus culture of Taxus baccata in relation to anticancer diterpenoid taxol biosynthesis. Biotechnology Letters (Chapman & Hall, London), vol. 18, N 11, pp. 1309-1314.

  16. Brukhin V.B., Bozhkov P.V. (1996) Female gametophyte development and embryogenesis in Taxus baccata L. – Acta Soc. Bot.Pol., vol. 65, N 1-2, pp. 135-139.

  17. Tchorzewska D., Brukhin V.B., Bednara J. (1996) Organelle layers at meiocyte of Psilotum nudum. – Acta Soc.Bot.Pol., vol. 65, N 1-2, pp. 91-96.

  18. Brukhin.V., Zemanek A. (1996) The manuscripts of Russian translations of the Polish herbal /Zielnik/ (1613) by Syreniusz in St. Petersburg.- Kwartalnik historii nauki i techniki (Warsaw), vol. 41, N 3-4, pp. 189-195.

  19. Brukhin V.B., Batygina T.B. (1994) Embryo culture and somatic embryogenesis in culture of Paeonia anomala L. – Phytomorphology. – v. 44, N 3 & 4. – pp. 151-157.

  20. Brukhin V.B. (2006) Histochemical and immunohistochemical aspects of embryogenesis. In: Embryology of flowering plants Terminology and Concepts. Ed. Prof. T.Batygina. Enfield, NH, USA, Science Publisher Inc. vol. 2 Seed, pp. 568-572.

  21. Batygina T.B., Brukhin V.B. (2006) Embryogenesis in Paeoniaceae. In: Embryology of flowering plants. Terminology and Concepts Ed. Prof. T.Batygina. Enfield, NH, USA, Science Publisher Inc. vol. 2 Seed, pp. 539-543.